AppleGFDB:The Apple Gene Function & Gene Family DataBase v1.0
Locus Search:

 

Expression

This Provides a tool to search expression leval of an apple gene. Please input an apple gene locus(e.g. MDP0000243428). Recently, molecular biological experiments of apple utilizing high-throughput hybridization array and sequencing-based techniques have become popular. In order to provide the user a convenient way to collect information about the expression of apple genes, we collected all available microarray informations from published papers to present in my database. To aggregate these datas into generalized events, it is necessary to define comprehensive expression profiles for apple researches. The expression pattern of apple genes would be an important cue for predict the function of unknown genes. Owing to that the knowledge of apple genes are lacking, the expression of apple genes can be functionally important for apple scientist. Our databases also offer a flexible and open design that facilitates submission, storage and retrieval of data sets from genomic hybridization and expression experiments. By supplying apple locus, the expression of the gene can be showed directly. To be specific, on the basis of the microarray analysis of apple maturation process, the expression of each gene in this process is visualized as bar graph in our database.

Platform: Transcriptome profiling of cultivar-specific apple fruit ripening and texture attributes

Input Locus
 

This Provides a tool to search expression leval of an apple gene during the apple ripening. Please input an apple gene locus(e.g. MDP0000243428).

Description: Molecular events regulating apple fruit ripening and sensory quality are largely unknown. Such knowledge is essential for genomic-assisted apple breeding and postharvest quality management. In this study, a parallel transcriptome profile analysis, scanning electron microscopic (SEM) examination and systematic physiological characterization were performed on two apple cultivars, Honeycrisp (HC) and Cripps Pink (CP), which have distinct ripening features and texture attributes. Systematic physiological characterization of fruit ripening based on weekly maturity data indicated substantial differences in fruit crispness and firmness at comparable ripening stages. SEM images of fruit cortex tissues prepared from fruits with equivalent maturity suggested that the cell wall thickness may contribute to the observed phenotypes of fruit firmness and crispness. A high-density long-oligo apple microarray consisting of duplex 190,135 cross-hybridization-free 50-70-mer isothermal probes, and representing 23,997 UniGene clusters, was manufactured on a Nimblegen array platform. Transcriptome profiling identified a total of 1793 and 1209 UniGene clusters differentially expressed during ripening from cortex tissues of HC and CP, respectively.

EBIID:E-GEOD-24523

Platform:A-GEOD-11164
Sample:
cultivar: Honeycrisp
Honeycrisp week 0
Developmental stage: week 0 relative to physiological maturity
Honeycrisp week -2
Developmental stage: week -2 relative to physiological maturity
Honeycrisp week -4
Developmental stage: week -4 relative to physiological maturity

cultivar: Cripps Pink
Cripps Pink week 0
Developmental stage: week 0 relative to physiological maturity
Cripps Pink week -2
Developmental stage: week -2 relative to physiological maturity
Cripps Pink week -4
Developmental stage: week -4 relative to physiological maturity

High-density DNA array prepared with Maskless Array Synthesizer (MAS) technology.
See manufacturer's website at http://www.nimblegen.com/

 

 


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